goat polyclonal antibody against cgrp  (Novus Biologicals)

Journal: iScience

Article Title: A novel Na v 1.8-FLPo driver mouse for intersectional genetics to uncover the functional significance of primary sensory neuron diversity

doi: 10.1016/j.isci.2024.109396

Figure Lengend Snippet:

Article Snippet: goat anti-CGRP , OriGene , cat# BP022.

Techniques: Recombinant, Electron Microscopy, Labeling, Plasmid Preparation, Mutagenesis, Cloning, Generated, Software

Journal: World Journal of Stem Cells

Article Title: Human amniotic fluid stem cell therapy can help regain bladder function in type 2 diabetic rats

doi: 10.4252/wjsc.v14.i5.330

Figure Lengend Snippet: Schema of experimental procedure. HFD: High-fat diet; IP: Intraperitoneal; STZ: Streptozotocin; ND: Normal diet; DM: Diabetes mellitus; hAFSCs: Human amniotic fluid stem cells; RT-PCR: Real-time polymerase chain reaction; M2: Muscarinic receptor 2; M3: Muscarinic receptor 3; NGF: Nerve growth factor; CGRP: Calcitonin gene-related peptide; PDX-1: Pancreatic-duodenal homeobox-1.

Article Snippet: After blocking with Dako REAL peroxidase blocking solution (S2023, DAKO Corp, Carpinteria, CA, United States) for 20 min, the sections were washed and incubated for 18-20 h at 4 °C with a rabbit polyclonal antibody against NGF (1:700, OriGene Technologies, Inc., Rockville, MD, United States), M2 (1:700, Millipore, Temecula, CA, United States), M3 (1:200, Santa Cruz Biotechnology, Santa Cruz, CA, United States), MafA (1:200, Abcam, Cambridge, MA, United States), or PDX-1 (1:100, Abcam), a goat polyclonal antibody against CGRP (1:200, Abcam), or a mouse monoclonal antibody against substance P (1:75, Abcam) or insulin (1:1200, Thermo Fisher Scientific Inc., Waltham, MA, United States).

Techniques: Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

Journal: World Journal of Stem Cells

Article Title: Human amniotic fluid stem cell therapy can help regain bladder function in type 2 diabetic rats

doi: 10.4252/wjsc.v14.i5.330

Figure Lengend Snippet: TaqMan probes used for quantitative polymerase chain reaction analysis

Article Snippet: After blocking with Dako REAL peroxidase blocking solution (S2023, DAKO Corp, Carpinteria, CA, United States) for 20 min, the sections were washed and incubated for 18-20 h at 4 °C with a rabbit polyclonal antibody against NGF (1:700, OriGene Technologies, Inc., Rockville, MD, United States), M2 (1:700, Millipore, Temecula, CA, United States), M3 (1:200, Santa Cruz Biotechnology, Santa Cruz, CA, United States), MafA (1:200, Abcam, Cambridge, MA, United States), or PDX-1 (1:100, Abcam), a goat polyclonal antibody against CGRP (1:200, Abcam), or a mouse monoclonal antibody against substance P (1:75, Abcam) or insulin (1:1200, Thermo Fisher Scientific Inc., Waltham, MA, United States).

Techniques: Real-time Polymerase Chain Reaction

Journal: World Journal of Stem Cells

Article Title: Human amniotic fluid stem cell therapy can help regain bladder function in type 2 diabetic rats

doi: 10.4252/wjsc.v14.i5.330

Figure Lengend Snippet: Immunoreactivities. In diabetes (DM) rats, the M2 immunoreactivity was increased at 4 wk compared with 12 wk. The M2 immunoreactivity was increased at 4 and 12 wk and M3 immunoreactivity was increased at 4 wk compared with normal diet (ND)/high-fat diet (HFD) control groups. The M2 immunoreactivity recovered at 4 wk after insulin treatment. The DM rats had significantly reduced immunoreactivities to nerve growth factor (NGF), calcitonin gene-related peptide (CGRP), and substance P at 4 and 12 wk compared with ND/HFD control groups. Insulin treatment can recover CGRP immunoreactivity and human amniotic fluid stem cells treatment can recover NGF immunoreactivity at 4 wk after DM induction. n = 6. Statistics: Median with first and third quartile (Q1, Q3) for continuous variables. Two-way analysis of variance was used for initial analysis. Kruskal-Wallis test with post hoc Bonferroni test for intergroup analysis. Mann-Whitney U test for the comparison between 4 wk and 12 wk. P values are shown in each comparison. A: M2-muscarinic receptor; B: M3-muscarinic receptor; C: Nerve growth factor; D: Calcitonin gene-related peptide; E: Substance P in the bladder of rats of different groups. ND: Normal diet; DM: Diabetes mellitus; hAFSCs: Human amniotic fluid stem cells; HFD: High-fat diet.

Article Snippet: After blocking with Dako REAL peroxidase blocking solution (S2023, DAKO Corp, Carpinteria, CA, United States) for 20 min, the sections were washed and incubated for 18-20 h at 4 °C with a rabbit polyclonal antibody against NGF (1:700, OriGene Technologies, Inc., Rockville, MD, United States), M2 (1:700, Millipore, Temecula, CA, United States), M3 (1:200, Santa Cruz Biotechnology, Santa Cruz, CA, United States), MafA (1:200, Abcam, Cambridge, MA, United States), or PDX-1 (1:100, Abcam), a goat polyclonal antibody against CGRP (1:200, Abcam), or a mouse monoclonal antibody against substance P (1:75, Abcam) or insulin (1:1200, Thermo Fisher Scientific Inc., Waltham, MA, United States).

Techniques: Control, MANN-WHITNEY, Comparison